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SmartGene GmbH bla oxa-14
Bla Oxa 14, supplied by SmartGene GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bla+oxa-14/bla+oxa+14/bio_rxiv__2021__09__20__461173-194-16-36
Average 90 stars, based on 1 article reviews
bla oxa-14 - by Bioz Stars, 2026-09
90/100 stars

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Expressing:

Article Title: Functional and Structural Characterization of OXA-935, a Novel OXA-10-family β-lactamase from Pseudomonas aeruginosa
Article Snippet: For the creation of OXA β-lactamase expression vectors for protein purification, both full length (FL) and mature sequence (trunc, Δaa1-20) bla OXA-14 (NCBI Reference Sequence WP_064056056.1) and bla OXA-935 (NCBI Reference Sequence WP_141989064.1) were codon optimized (SmartGene®) for expression in E. coli , synthesized (Twist Bioscience) and cloned into the pMCSG53 vector , which contains a tobacco etch virus (TEV) cleavable N-terminal 6x His-tag, ampicillin resistance and genes for rare codons.

Protein Purification:

Article Title: Functional and Structural Characterization of OXA-935, a Novel OXA-10-family β-lactamase from Pseudomonas aeruginosa
Article Snippet: For the creation of OXA β-lactamase expression vectors for protein purification, both full length (FL) and mature sequence (trunc, Δaa1-20) bla OXA-14 (NCBI Reference Sequence WP_064056056.1) and bla OXA-935 (NCBI Reference Sequence WP_141989064.1) were codon optimized (SmartGene®) for expression in E. coli , synthesized (Twist Bioscience) and cloned into the pMCSG53 vector , which contains a tobacco etch virus (TEV) cleavable N-terminal 6x His-tag, ampicillin resistance and genes for rare codons.

Sequencing:

Article Title: Functional and Structural Characterization of OXA-935, a Novel OXA-10-family β-lactamase from Pseudomonas aeruginosa
Article Snippet: For the creation of OXA β-lactamase expression vectors for protein purification, both full length (FL) and mature sequence (trunc, Δaa1-20) bla OXA-14 (NCBI Reference Sequence WP_064056056.1) and bla OXA-935 (NCBI Reference Sequence WP_141989064.1) were codon optimized (SmartGene®) for expression in E. coli , synthesized (Twist Bioscience) and cloned into the pMCSG53 vector , which contains a tobacco etch virus (TEV) cleavable N-terminal 6x His-tag, ampicillin resistance and genes for rare codons.

Synthesized:

Article Title: Functional and Structural Characterization of OXA-935, a Novel OXA-10-family β-lactamase from Pseudomonas aeruginosa
Article Snippet: For the creation of OXA β-lactamase expression vectors for protein purification, both full length (FL) and mature sequence (trunc, Δaa1-20) bla OXA-14 (NCBI Reference Sequence WP_064056056.1) and bla OXA-935 (NCBI Reference Sequence WP_141989064.1) were codon optimized (SmartGene®) for expression in E. coli , synthesized (Twist Bioscience) and cloned into the pMCSG53 vector , which contains a tobacco etch virus (TEV) cleavable N-terminal 6x His-tag, ampicillin resistance and genes for rare codons.

Clone Assay:

Article Title: Functional and Structural Characterization of OXA-935, a Novel OXA-10-family β-lactamase from Pseudomonas aeruginosa
Article Snippet: For the creation of OXA β-lactamase expression vectors for protein purification, both full length (FL) and mature sequence (trunc, Δaa1-20) bla OXA-14 (NCBI Reference Sequence WP_064056056.1) and bla OXA-935 (NCBI Reference Sequence WP_141989064.1) were codon optimized (SmartGene®) for expression in E. coli , synthesized (Twist Bioscience) and cloned into the pMCSG53 vector , which contains a tobacco etch virus (TEV) cleavable N-terminal 6x His-tag, ampicillin resistance and genes for rare codons.

Plasmid Preparation:

Article Title: Functional and Structural Characterization of OXA-935, a Novel OXA-10-family β-lactamase from Pseudomonas aeruginosa
Article Snippet: For the creation of OXA β-lactamase expression vectors for protein purification, both full length (FL) and mature sequence (trunc, Δaa1-20) bla OXA-14 (NCBI Reference Sequence WP_064056056.1) and bla OXA-935 (NCBI Reference Sequence WP_141989064.1) were codon optimized (SmartGene®) for expression in E. coli , synthesized (Twist Bioscience) and cloned into the pMCSG53 vector , which contains a tobacco etch virus (TEV) cleavable N-terminal 6x His-tag, ampicillin resistance and genes for rare codons.

Virus:

Article Title: Functional and Structural Characterization of OXA-935, a Novel OXA-10-family β-lactamase from Pseudomonas aeruginosa
Article Snippet: For the creation of OXA β-lactamase expression vectors for protein purification, both full length (FL) and mature sequence (trunc, Δaa1-20) bla OXA-14 (NCBI Reference Sequence WP_064056056.1) and bla OXA-935 (NCBI Reference Sequence WP_141989064.1) were codon optimized (SmartGene®) for expression in E. coli , synthesized (Twist Bioscience) and cloned into the pMCSG53 vector , which contains a tobacco etch virus (TEV) cleavable N-terminal 6x His-tag, ampicillin resistance and genes for rare codons.



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SmartGene GmbH bla oxa-14
Bla Oxa 14, supplied by SmartGene GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bla+oxa-14/bla+oxa+14/bio_rxiv__2021__09__20__461173-194-16-36
Average 90 stars, based on 1 article reviews
bla oxa-14 - by Bioz Stars, 2026-09
90/100 stars
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